Coming soon:
Slabodnick MM, Tintori SC, Prakash M, Higgins CD, Chen AH, Cupp TD, Wong T, Bowie E, Jug F, Goldstein B. Afadin and zyxin contribute to coupling between cell junctions and contractile actomyosin networks during apical constriction. Submitted & preprinted.
Hibshman JD, Carra S, Goldstein B. Tardigrade small heat shock proteins can limit desiccation-induced protein aggregation. Submitted & preprinted.
Published articles and reviews:
Goldstein B, Srivastava, M., eds. (2022) Emerging Model Systems in Developmental Biology, Academic Press. (a volume with chapters by scientists who have developed new model organisms and/or advanced tools for emerging models, in the Current Topics in Developmental Biology series)
Goldstein B (2022). Tardigrades and their emergence as model organisms, in B Goldstein and M Srivastava, Emerging Model Systems in Developmental Biology (pp 173-198), Academic Press.
lin-12 ICD Notch Pani
Pani AM, Gibney TV, Medwig-Kinney TN, Matus DQ, Goldstein B (2022). A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans. microPublication Biology https://doi.org/10.17912/micropub.biology.000603.
Goldstein B (2022) Tardigrades. Nature Methods 19:904-905.
Giovannini I, Boothby TC, Cesari M, Goldstein B, Guidetti R, Rebecchi L (2022). Production of reactive oxygen species and involvement of bioprotectants during anhydrobiosis in the tardigrade Paramacrobiotus spatialis. Scientific Reports 12:1938.​
Jon Hibshman C. elegans LEA-1 desiccation goldstein lab
Hibshman JD, Goldstein B (2021). LEA motifs promote desiccation tolerance in vivo. BMC Biology, 19:263. https://doi.org/10.1186/s12915-021-01176-0.
Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD (2021). An expanded auxin-inducible degron toolkit for Caenorhabditis elegans. Genetics 217(3) iyab006 https://doi.org/10.1093/genetics/iyab006
Goldstein B, Avasthi P (2021). A Guide to Setting Up and Managing a Lab at a Research-Intensive Institution. BMC Proceedings 15(Suppl 2):8. https://doi.org/10.1186/s12919-021-00214-7
Goldstein B (2021). A Lab of Her Own. Nautilus 41: 20-33.
Hibshman JD, Clegg JS, Goldstein B (2020). Mechanisms of Desiccation Tolerance: Themes and Variations in Brine Shrimp, Roundworms, and Tardigrades. Frontiers in Physiology 11:592016.
Sophie Tintori Patrick Golden DrEdge tool gene visualization
Tintori SC, Golden P, Goldstein B (2020). Differential Expression Gene Explorer (DrEdGE): A tool for generating interactive online visualizations of gene expression datasets. Bioinformatics 36:2581-2583.
* Access the DrEdGE tool here to see how it works and build your own web-based gene expression data visualization *
Goldstein B, Nance J (2020). C. elegans gastrulation: a model for understanding how cells polarize, change shape, and journey toward the center of an embryo. Genetics 214:265-277.
Conradt B, Claycomb JM, Goldstein B, Shen K, Oegema K, Desai A, Colón-Ramos DA, Murphy CT, Sherwood DR, Walhout M (2019). The Power of C. elegans: A Tribute to Sydney Brenner. Developmental Cell 49:496-498.
Gordon KL, Payne SG, Linden-High LM, Pani AM, Goldstein B, Hubbard EJA, Sherwood DR (2019). Ectopic germ cells can induce niche-like enwrapment by neighboring body wall muscle. Current Biology 29:823-833.
Ari Ariel Pani eLife Wnt C. elegans
Pani AM and B Goldstein (2018). Direct visualization of a native Wnt in vivo reveals that a long-range Wnt gradient forms by extracellular dispersal.  eLife 7:e38325.
Frank Smith water bear tardigrade brain
Smith FW, Cumming M, Goldstein B (2018). Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains. EvoDevo 9:19.
Jenny Jennifer Heppert 2018 Genetics
Heppert JK, Pani AM, Roberts AM, Dickinson DJ, and B Goldstein (2018). A CRISPR tagging-based screen reveals localized players in Wnt-directed asymmetric cell division. Genetics 208:1147-1164.
Goldstein B (2018). The emergence of the tardigrade Hypsibius exemplaris as a model system. CSH Protocols, 10:859-866.
Kristen McGreevy hypsibius exemplaris fluorescent
McGreevy KM, Heikes KL, Kult S, Tharp ME, and B Goldstein (2018). Fluorescent cell staining methods for living Hypsibius exemplaris embryos. CSH Protocols 10:878-884.
Heikes KL and B Goldstein (2018). Live imaging of tardigrade embryonic development by differential interference contrast microscopy. CSH Protocols 10:974-877.
Fielmich LE, Schmidt R, Dickinson DJ, Goldstein B, Akhmanova A, and S Van den Heuvel (2018). Optogenetic dissection of mitotic spindle positioning in vivo. eLife 7:e38199.
Goldstein B (2018). On Francis Crick, the genetic code, and a clever kid. Current Biology 28:R305.
Dickinson DJ, Slabodnick MM, Chen AH, and Goldstein B (2018). SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette. Micropublication: biology. Dataset. https://doi.org/10.17912/W2KT0N
Yumerefendi H, Wang H, Dickinson DJ, Lerner A, Malkus P, Goldstein B, Hahn K, and Kuhlman B (2018). Light-dependent cytoplasmic recruitment enhances the dynamic range of a nuclear import photoswitch. Chembiochem. 19:1319-1325.
Fadero TC, Gerbich TM, Rana K, Suzuki A, DiSalvo M, Schaefer KN, Heppert JK, Boothby TC, Goldstein B, Peifer M, Allbritton NL, Gladfelter AS, Maddox AS and Maddox PS (2018). LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching. Journal of Cell Biology 217:1869-1882.
Martinez P, Allsman LA, Brakke KA, Hoyt C, Hayes J, Liang H, Neher W, Rui Y, Roberts AM, Moradifam A, Goldstein B, Anderson CT, Rasmussen C (2018). Predicting division planes of three-dimensional cells by soap-film minimization. Plant Cell 30:2255-2266.
single cell biochemistry Dan Dickinson
Dickinson DJ, Schwaeger F, Pintard L, Gotta M, and B Goldstein (2017). A Single-Cell Biochemistry Approach Reveals PAR Complex Dynamics during Cell Polarization. Developmental Cell 42:416-434.
hypsibius exemplaris Boothby water bear IDPs
Boothby TC, Tapia H, Brozena AH, Piszkiewicz S, Smith AE, Giovanninni I, Rebecchi L, Pielak GJ, Koshland D, and B Goldstein (2017). Tardigrades use intrinsically disordered proteins to survive desiccation. Molecular Cell 65:975-984.
hypsibius exemplaris tardigrade brain Frank Smith
Smith FW, Bartels PJ, and Goldstein B (2017). A hypothesis for the composition of the tardigrade brain and its implications for panarthropod brain evolution. Integrative and Comparative Biology 57:546-559.
Smith FW and B Goldstein (2017). Segmentation in Tardigrada and diversification of segmental patterns in Panarthropoda. Arthropod Structure & Development 46:328-340.
Zallen JA and Goldstein B (2017). Cellular mechanisms of morphogenesis. Semin Cell Dev Biol 67:101-102.
Russell JJ, Theriot JA, Sood P, Marshall WF, Landweber LF, Fritz-Laylin L, Polka JK, Oliferenko S, Gerbich T, Gladfelter A, Umen J, Bezanilla M, Lancaster MA, He S, Gibson MC, Goldstein B, Tanaka EM, Hu C-K, and Brunet A (2017). Non-model model organisms. BMC Biology 15:55.
Goldstein B and Zallen JA (2017). Cell Polarity and Morphogenesis: new technologies and new findings. Molecular Biology of the Cell, 28:699-700.
Naegeli KM, Hastie E, Garde A, Wang Z, Keeley DP, Gordon KL, Pani AM, Kelley LC, Morrissey MA, Chi Q, Goldstein B, Sherwood DR (2017). Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain. Developmental Cell 43:403–417.
Linden LM, Gordon KL, Pani AM, Payne SG, Garde A, Burkholder D, Chi Q, Goldstein B and Sherwood DR (2017). Identification of regulators of germ stem cell enwrapment by its niche in C. elegans. Developmental Biology 429:271-284.
Ladouceur AM, Ranjan R, Smith L, Fadero T, Heppert J, Goldstein B, Maddox AS, Maddox PS (2017). CENP-A and topoisomerase-II antagonistically affect chromosome length. Journal of Cell Biology 216:2645-2655.
Heppert fluorescent proteins in vivo
Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer J, Kuhn JR, and B Goldstein (2016). Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system. Molecular Biology of the Cell 27:3385-3394. (and a spectrum viewer tool)
Picture
Tintori SC, Osborne Nishimura E, Golden P,  Lieb JD, and Goldstein B (2016). A transcriptional lineage of the early C. elegans embryo. Developmental Cell 38:430-444.
(and an interactive data visualization tool)
Hypsibius exemplaris tardigrade hox
Smith FW, Boothby TC, Giovannini I, Rebecchi L, Jockusch EL, and B Goldstein (2016). The compact body plan of tardigrades evolved by the loss of a large body region. Current Biology 26:224-229.
MRCK apical constriction
Marston DJ*, Higgins CD*, Peters KA, Cupp TD, Dickinson DJ, Pani AM, Moore RP, Cox AH, Kiehart DP, and Goldstein B (2016). MRCK-1 drives apical constriction in C. elegans by linking developmental patterning to force generation. Current Biology 26:2079-2089.
neural tube closure animal models
Sullivan-Brown J, Tandon P, Bird KE, Dickinson DJ, Tintori SC, Heppert JK, Meserve JH, Trogden KP, Orlowski SK, Conlon FL, and Goldstein B (2016). Identifying regulators of morphogenesis common to vertebrate neural tube closure and C. elegans gastrulation. Genetics 202:123-139.
Heppert JK and Goldstein B (2016). Remodelling germ cells by intercellular cannibalism. Nature Cell Biology 18:1267-1268.
Goldstein B and King N (2016). The Future of Cell Biology: Emerging Model Organisms. Trends in Cell Biology 11:818-824.
Goldstein B (2016). Sydney Brenner on the Genetics of Caenorhabditis elegans. Genetics 204:1-2.
Dickinson DJ and Goldstein B (2016). CRISPR-based methods for Caenorhabditis elegans genome engineering. Genetics 202:885-901.
CRISPR Dickinson
Dickinson, DJ, AM Pani, JK Heppert, CD Higgins, and B Goldstein (2015). Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035-1049.
Das A, Dickinson DJ, Wood CC, Goldstein B, and Slep KC (2015). Crescerin uses a TOG domain array to regulate microtubules in the primary cilium. Mol Biol Cell 26:4248-64.
Hypsibius exemplaris
Boothby TC, Tenlen JR, Smith FW, Wang JR, Patanella KA, Osborne Nishimura E, Tintori SC, Li Q, Jones CD, Yandell M, Messina DN, Glasscock J, and Goldstein B (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. PNAS 112:15976-15981 and follow-up letter.
Goldstein, B and DP Kiehart (2015). Moving Inward: Establishing the Mammalian Inner Cell Mass. Developmental Cell 34:385-386.
Yumerefendi H, Dickinson DJ, Wang H, Zimmerman SP, Bear JE, Goldstein B, Hahn K, and Kuhlman B (2015). Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS One 10(6):e0128443.
Osborne Nishimura E, Zhang JC, Werts AD, Goldstein B, Lieb JD (2015). Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis. PLoS Genetics 11(4):e1005117.
Sarkies P, Selkirk ME, Jones JT, Blok V, Boothby T, Goldstein B, Hanelt B, Ardila-Garcia A, Fast NM, Schiffer PM, Kraus C, Taylor MJ, Koutsovoulos G, Blaxter ML, and Miska EA (2015). Ancient and Novel Small RNA Pathways Compensate for the Loss of piRNAs in Multiple Independent Nematode Lineages. PLoS Biology 13(2):e1002061.
Goldstein B (2015). The Thrill of Defeat: What Francis Crick and Sydney Brenner taught me about being scooped. Nautilus online, Feb 26 2015, updated and reprinted May 30, 2019.
Martin, A.C. and B. Goldstein (2014). Apical constriction: themes and variations on a cellular mechanism driving morphogenesis. Development 141:1987-1998.
CRISPR C. elegans
Dickinson, D.J., J.D. Ward, D.J. Reiner and B. Goldstein (2013). Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nature Methods 10:1028-1034.
Peters EC, Gossett AJ, Goldstein B, Der CJ, Reiner DJ (2013). Redundant Canonical and Noncanonical Caenorhabditis elegans p21-Activated Kinase Signaling Governs Distal Tip Cell Migrations. G3 3:181-195.
Gao, L., L. Shao, C.D. Higgins, J.S. Poulton, M. Peifer, M.W. Davidson,  X. Wu, B. Goldstein and E. Betzig (2012). Noninvasive imaging of 3D dynamics in thickly fluorescent specimens beyond the diffraction limit. Cell 151:1370-1385.

Sullivan-Brown, J. and B. Goldstein (2012) Neural tube closure: The curious case of shrinking junctions. Current Biology 22:R574-R576.

Edgar, L.G. and B. Goldstein (2012). Culture and Manipulation of Embryonic Cells, in Caenorhabditis elegans: Cell Biology and Physiology, eds. Joel H. Rothman and Andrew Singson. Methods in Cell Biology 107:151-175.

Tse, Y.C., M. Werner, K.M. Longhini, J.-C. Labbé, B. Goldstein and M. Glotzer (2012) RhoA activation during polarization and cytokinesis of the early C. elegans embryo are differentially dependent on NOP-1 and CYK-4. Molecular Biology of the Cell 23:4020-4031.
Goldstein, B. (2011) Primer: Visualizing with ImageJ. Make 27:116-121. (This is a magazine article that introduces non-scientists to ImageJ, encouraging hobbyists to write new ImageJ plugins that might benefit science) and a 2018 online update of the article.
Werts, A.D. and B. Goldstein (2011) How signaling between cells can orient a mitotic spindle. Seminars in Cell and Developmental Biology 22:842-849.

Higgins C.D. and B. Goldstein (2010) Asymmetric Cell Division: A New Way to Divide Unequally. Current Biology R1029-1031.

Sawyer, J.M., J.R. Harrell, G. Shemer, J. Sullivan-Brown, M. Roh-Johnson, and B. Goldstein (2010) Apical constriction: A cell shape change that can drive morphogenesis. Developmental Biology 341:5-19.

Roh-Johnson, M., J. Sullivan-Brown, and B. Goldstein (2010) Roles for Actin Dynamics in Cell Movements During Development. Chapter in Actin-Based Motility: Cellular, Molecular and Physical Aspects, ed. M.-F. Carlier, Springer.

Arata, Y., J.-Y. Lee, B. Goldstein, and H. Sawa (2010) Extracellular control of PAR protein localization during asymmetric cell division in the C. elegans embryo. Development 137:3337-3345.
Goldstein, B. and H. Hamada (2009) Shape Meets Polarity in Japan. Development 136: 2487-2492.

Marston, D.J., M. Roh, A. Mikels, R. Nusse, and B. Goldstein (2008) Wnt signaling during Caenorhabditis elegans embryonic development. Methods in Molecular Biology 469: 103-111.

Goldstein, B. and I.G. Macara (2007) The PAR Proteins: Fundamental Players in Animal Cell Polarization. Developmental Cell 13: 609-622.
Marston, D.J. and B. Goldstein (2006) Symmetry Breaking in C. elegans: Another Gift from the Sperm. Developmental Cell 11: 273-274. 
Marston, D.J. and B. Goldstein (2006) Actin-based forces driving embryonic morphogenesis in C. elegans. Current Opinion in Genetics and Development 16: 392-398.
McCarthy, E.K. and B. Goldstein (2006) Asymmetric Spindle Positioning. Current Opinion in Cell Biology 18: 79-85.

Nance, J., J.-Y. Lee, and B. Goldstein (2005) Gastrulation in C. elegans. WormBook, ed. The C. elegans Research Community.

McCarthy, E.K. and B. Goldstein (2005) Asymmetric Division: A Kinesin for Spindle Positioning. Current Biology 15: R591-593.

Dudley, N.R., A.Z. Amin, and B. Goldstein (2005) Genes Required for RNA Interference, pp 55-68 in RNA Interference Technology: From Basic Science to Drug Development, edited by K. Appasani (Cambridge University Press).

Dudley, N.R. and B. Goldstein (2005) RNA Interference in Caenorhabditis elegans. Chapter in RNA Silencing: Methods and Protocols, edited by G. Carmichael (Humana Press), Methods in Molecular Biology 309:29-38.
Goldstein, B. (2003) Asymmetric Division: AGS Proteins Position the Spindle. Current Biology 13: R879-R880.
Dudley, N.R. and B. Goldstein (2003) RNA interference: Silencing in the cytoplasm and nucleus. Curr Opin Mol Ther 5:113-117.
Labbé, J.-C. and B. Goldstein (2002) Embryonic Development: A New SPN on Cell Fate Specification. Current Biology 12:R396-R398.

Goldstein, B. and M. Blaxter (2002) Tardigrades. Current Biology 12: R475.

Goldstein, B. (2001) On the Evolution of Early Development in the Nematoda.  Phil Trans Royal Society B 356: 1521-1531.
Goldstein B. (2000) The Professional Debunker (review of the book Voodoo Science: the Road from Foolishness to Fraud, by Robert L. Park), Nature Cell Biology 2:E212.

Goldstein, B. (2000) Embryonic polarity: A role for microtubules. Current Biology 10:R820-R822.

Goldstein, B. (2000) When cells tell their neighbors which direction to divide. Developmental Dynamics 218:23-29.
Wittmann, C., O. Bossinger, B. Goldstein, M. Fleischmann, R. Kohler, K. Brunschwig, H. Tobler, and F. Müller (1997) The expression of the C. elegans labial-like Hox gene ceh-13 during early embryogenesis relies on cell fate and on anteroposterior cell polarity. Development 124: 4193-4200.

Goldstein, B. and G. Freeman (1997) Axis specification in animal development. BioEssays 19: 105-116.
Goldstein, B., S. N. Hird, and J. G.White (1993) Cell polarity in early C. elegans development. Development 1993 Supplement: 279-287.
Reviews of our work:
Faculty Opinions reviews of "Direct visualization of a native Wnt in vivo reveals that a long-range Wnt gradient forms by extracellular dispersal"

An interview with Dan Dickinson at Development's web site, The Node, about his paper in Developmental Cell

"Protein Clustering Shapes Polarity Protein Gradients", a Developmental Cell Preview article about Dickinson et al. 2017 and two other papers

Faculty of 1000 review of "A Single-Cell Biochemistry Approach Reveals PAR Complex Dynamics during Cell Polarization"

Faculty of 1000 review of "Tardigrades use intrinsically disordered proteins to survive desiccation"

"Intrinsically Disordered Proteins Adaptively Reorganize Cellular Matter During Stress", a Trends in Biochemical Sciences article about Boothby et al. 2017

"Meet the author" interview with Thomas Boothby in Molecular Cell

"Meet the author" interview with Sophie Tintori in Developmental Cell

Faculty of 1000 review of "MRCK-1 drives apical constriction in C. elegans by linking developmental patterning to force generation"

"Zoology: The Walking Heads", a Current Biology dispatch about Smith et al. 2016

Highlight in Genetics of "Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation"

ASCB Profile of Bob

Highlight in Genetics of "Streamlined genome engineering with a self-excising drug selection cassette"

Inside Life Science article from NIGMS about recent cell migration findings including ours

Interview with Bob in The Journal of Cell Biology

Faculty of 1000 review of "RNA interference can be used to disrupt gene function in tardigrades"

"Cell Biology: Embryonic Clutch Control", by William Razzell and Paul Martin in Science

Faculty of 1000 reviews of "Triggering a Cell Shape Change by Exploiting Pre-Existing Actomyosin Contractions"

"Cell Change Up", a review of "Triggering a Cell Shape Change by Exploiting Pre-Existing Actomyosin Contractions" in The Scientist

Faculty of 1000 review of "Dynamic localization of C. elegans TPR-GoLoco proteins mediates mitotic spindle orientation by extrinsic signaling"

"TPR-GoLoco moves to orientate division", an In This Issue highlight about Werts et al (2011) in Development

Genetics highlight of "Overcoming Redundancy: an RNAi Enhancer Screen for Morphogenesis Genes in Caenorhabditis elegans"

Faculty of 1000 review of "Overcoming Redundancy: an RNAi Enhancer Screen for Morphogenesis Genes in Caenorhabditis elegans"

Faculty of 1000 review of "A Cell Cycle Timer for Asymmetric Spindle Positioning"
Faculty of 1000 review of "The Tardigrade Hypsibius dujardini, a New Model for Studying the Evolution of Development"

Faculty of 1000 review of "Segmental Expression of Pax3/7 and Engrailed Homologs in Tardigrade Development"

"Gastrulation: Wnts Signal Constriction", a Dispatch article about Lee et al. 2006, from Current Biology

Faculty of 1000 reviews of "Wnt/Frizzled Signaling Controls C. elegans Gastrulation by Activating Actomyosin Contractility"

"Tugging and pulling in asymmetric cell divisions" from The Journal of Cell Biology

Faculty of 1000 reviews of "Wnt Signals Can Function as Positional Cues in Establishing Cell Polarity"

"Bringing classical embryology to C. elegans gastrulation", a Preview article written by our hero Eric Wieschaus and colleagues, from Developmental Cell
 
"Microtubule cytoskeleton: Navigating the intracellular landscape", a Dispatch article about Labbé et al 2003 and four other new papers, from Current Biology

 "Gastrulation: Partaking of the bottle", a Dispatch article about Lee & Goldstein 2003 and one other paper, from Current Biology

Faculty of 1000 reviews of "Mechanisms of cell positioning during C. elegans gastrulation"

"New & Notable: RNA Interference", from The Scientist

Faculty of 1000 reviews of "Using RNA Interference to Identify Genes Required for RNA Interference"

"UNC's Bob Goldstein talks about getting into RNAi and hunting for genes", from RNAi News

 "RNAi to RNAi", from Genome Biology
Popular press, blogs:

NPR
tardigrades in space
Tardigrade space project begun in the lab in 2014 by then-postdoc Thomas Boothby has arrived at the International Space Station
- NASA story

NPR and NY Times
water bear
NPR and NY Times stories about postdoc Thomas Boothby and colleagues' discoveries about disordered proteins

Discover Magazine blog
water bear
"Tardigrades Are One Giant Head", a story about our evo-devo work using tardigrades

NPR Science Friday
water bear
"Behold The Mighty, Microscopic Water Bear", a video about our evo-devo work using tardigrades

Wired
water bears
a Wired Science story about extreme survival of water bears and our work

National Geographic Channel
water bears
a National Geographic Channel clip of us talking about tardigrades

Gig Posters for Scientists
biology gig posters bob goldstein
Some stories about the posters
- The Scientist, 2009
- Fast Company, 2019

Endeavors Magazine
water bears
"Tardi-whats?", a story about our evo-devo work using tardigrades


Science
science sciencemag goldstein elegans videos
"Cannes of Worms", about our C. elegans Movies web site, from Science 295:2331 (2002)
 
Cover images:

(*co-first authors)