Publications

Coming soon:

Goldstein B. Animal Physiology: How do tardigrades survive intense irradiation? Current Biology, in press.

Heikes KL, Goldstein B. Expression patterns of FGF and BMP pathway genes in the tardigrade Hypsibius exemplaris. bioRxiv 2024.01.29.577774; doi: https://doi.org/10.1101/2024.01.29.577774

Pani AM, Favichia M, Goldstein B. Long-distance Wnt transport in axons highlights cell type-specific modes of Wnt transport in vivo. bioRxiv 2023.05.03.539245; doi.org/10.1101/2023.05.03.539245

Published articles and reviews:

Zhang et al apical constriction basolateral Arp2/3 WAVE Rac signaling

Zhang P, Medwig-Kinney T, Breiner EA, Perez JM, Song AN, Goldstein B (2025). Cell signaling facilitates apical constriction by basolaterally recruiting Arp2/3 via Rac and WAVE. Journal of Cell Biology 224(5):e202409133.

Hibshman JD, Clark-Hachtel CM, Bloom KS, Goldstein B (2024). A bacterial expression cloning screen reveals single-stranded DNA-binding proteins as potent desicco-protectants. Cell Reports 43:114956. doi 10.1016/j.celrep.2024.114956.

Harry CJ*, Hibshman JD*, Damatac A, Davidson PL, Estermann MA, Flores-Flores M, Holmes CM, Lázaro J, Legere EA, Leyhr J, Thendral SB, Vincent BA, Goldstein B (2024). Protocol for fluorescent live-cell staining of tardigrades. STAR Protocols 5:103232.

Clark-Hachtel CM, Hibshman JD, De Buysscher T, Stair ER, Hicks LM, Goldstein B (2024). The tardigrade Hypsibius exemplaris dramatically upregulates DNA repair pathway genes in response to ionizing radiation. Current Biology 34:1819-1830.

apical constriction C. elegans Pu Zhang Taylor Medwig-Kinney

Zhang P*, Medwig-Kinney TN*, Goldstein B (2023). Architecture of the cortical actomyosin network driving apical constriction in C. elegans. Journal of Cell Biology 222(9):e202302102. doi.org/10.1083/jcb.202302102

apical constriction zyxin adherens junctions C. elegans

Slabodnick MM*, Tintori SC*, Prakash M, Zhang P, Higgins CD, Chen AH, Cupp TD, Wong T, Bowie E, Jug F, Goldstein B (2023). Zyxin contributes to coupling between cell junctions and contractile actomyosin networks during apical constriction. PLoS Genetics 19(3): e1010319. doi.org/10.1371/journal.pgen.1010319

Serre JM, Slabodnick MM, Goldstein B, Hardin J (2023). SRGP-1/srGAP and AFD-1/afadin stabilize HMP-1/alpha-catenin at rosettes to seal internalization sites following gastrulation in C. elegans. PLoS Genetics 19(3):e1010507 doi.org/10.1371/journal.pgen.1010507

Costa DS, Kenny-Ganzert IW, Chi Q, Park K, Kelley LC, Garde A, Matus DQ, Park J, Yogev S, Goldstein B, Gibney TV, Pani AM, Sherwood DR (2023). The C. elegans Anchor Cell Transcriptome: Ribosome Biogenesis Drives Cell Invasion through Basement Membrane. Development 150(9):dev201570.

Heikes KL, Game M, Smith FW, Goldstein B (2023). The Embryonic Origin of Primordial Germ Cells in the Tardigrade Hypsibius exemplaris. Developmental Biology 497:42-58.

sHSP small heat shock proteins tardigrade hypsibius hibshman

Hibshman JD, Carra S, Goldstein B (2023). Tardigrade small heat shock proteins can limit desiccation-induced protein aggregation. Communications Biology, 6:121 doi:10.1038/s42003-023-04512-y.

Pani AM, Gibney TV, Medwig-Kinney TN, Matus DQ, Goldstein B (2022). A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans. microPublication Biology https://doi.org/10.17912/micropub.biology.000603.

Lynch AM, Zhu Y, Lucas BG, Winkelman JD, Bai K, Martin SCT, Block S, Slabodnick MM, Audhya A, Goldstein B, Pettitt J, Gardel ML, Hardin J (2022). TES-1/Tes and ZYX-1/Zyxin protect junctional actin networks under tension during epidermal morphogenesis in the C. elegans embryo. Current Biology 32:5189-5199.

Goldstein B, Srivastava, M., eds. (2022). Emerging Model Systems in Developmental Biology, Academic Press. (a volume with chapters by scientists who have developed new model organisms and/or advanced tools for emerging models, in the Current Topics in Developmental Biology series)

Goldstein B (2022). Tardigrades and their emergence as model organisms, in B Goldstein and M Srivastava, Emerging Model Systems in Developmental Biology (pp 173-198), Academic Press.

Goldstein B (2022). Tardigrades. Nature Methods 19:904-905.

Giovannini I, Boothby TC, Cesari M, Goldstein B, Guidetti R, Rebecchi L (2022). Production of reactive oxygen species and involvement of bioprotectants during anhydrobiosis in the tardigrade Paramacrobiotus spatialis. Scientific Reports 12:1938.

Jon Hibshman C. elegans LEA-1 desiccation goldstein lab

Hibshman JD, Goldstein B (2021). LEA motifs promote desiccation tolerance in vivo. BMC Biology, 19:263. https://doi.org/10.1186/s12915-021-01176-0 .

Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD (2021). An expanded auxin-inducible degron toolkit for Caenorhabditis elegans. Genetics 217(3) iyab006 https://doi.org/10.1093/genetics/iyab006

Goldstein B, Avasthi P (2021). A Guide to Setting Up and Managing a Lab at a Research-Intensive Institution. BMC Proceedings 15(Suppl 2):8. https://doi.org/10.1186/s12919-021-00214-7

Goldstein B (2021). A Lab of Her Own. Nautilus 41: 20-33.

Hibshman JD, Clegg JS, Goldstein B (2020). Mechanisms of Desiccation Tolerance: Themes and Variations in Brine Shrimp, Roundworms, and Tardigrades. Frontiers in Physiology 11:592016.

Sophie Tintori Patrick Golden DrEdge tool gene visualization

Tintori SC, Golden P, Goldstein B (2020). Differential Expression Gene Explorer (DrEdGE): A tool for generating interactive online visualizations of gene expression datasets. Bioinformatics 36:2581-2583.
* Access the DrEdGE tool here to see how it works and build your own web-based gene expression data visualization *

Goldstein B, Nance J (2020). C. elegans gastrulation: a model for understanding how cells polarize, change shape, and journey toward the center of an embryo. Genetics 214:265-277.

Conradt B, Claycomb JM, Goldstein B, Shen K, Oegema K, Desai A, Colón-Ramos DA, Murphy CT, Sherwood DR, Walhout M (2019). The Power of C. elegans: A Tribute to Sydney Brenner. Developmental Cell 49:496-498.

Gordon KL, Payne SG, Linden-High LM, Pani AM, Goldstein B, Hubbard EJA, Sherwood DR (2019). Ectopic germ cells can induce niche-like enwrapment by neighboring body wall muscle. Current Biology 29:823-833.

Pani AM and B Goldstein (2018). Direct visualization of a native Wnt in vivo reveals that a long-range Wnt gradient forms by extracellular dispersal. eLife 7:e38325.

Smith FW, Cumming M, Goldstein B (2018). Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains. EvoDevo 9:19.

Heppert JK, Pani AM, Roberts AM, Dickinson DJ, and B Goldstein (2018). A CRISPR tagging-based screen reveals localized players in Wnt-directed asymmetric cell division. Genetics 208:1147-1164.

Goldstein B (2018). The emergence of the tardigrade Hypsibius exemplaris as a model system. CSH Protocols, 10:859-866.

Kristen McGreevy hypsibius exemplaris fluorescent

McGreevy KM, Heikes KL, Kult S, Tharp ME, and B Goldstein (2018). Fluorescent cell staining methods for living Hypsibius exemplaris embryos. CSH Protocols 10:878-884.

Heikes KL and B Goldstein (2018). Live imaging of tardigrade embryonic development by differential interference contrast microscopy. CSH Protocols 10:974-877.

Fielmich LE, Schmidt R, Dickinson DJ, Goldstein B, Akhmanova A, and S Van den Heuvel (2018). Optogenetic dissection of mitotic spindle positioning in vivo. eLife 7:e38199.

Goldstein B (2018). On Francis Crick, the genetic code, and a clever kid. Current Biology 28:R305.

Dickinson DJ, Slabodnick MM, Chen AH, and Goldstein B (2018). SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette. microPublication Biology. https://doi.org/10.17912/W2KT0N

Yumerefendi H, Wang H, Dickinson DJ, Lerner A, Malkus P, Goldstein B, Hahn K, and Kuhlman B (2018). Light-dependent cytoplasmic recruitment enhances the dynamic range of a nuclear import photoswitch. Chembiochem. 19:1319-1325.

Fadero TC, Gerbich TM, Rana K, Suzuki A, DiSalvo M, Schaefer KN, Heppert JK, Boothby TC, Goldstein B, Peifer M, Allbritton NL, Gladfelter AS, Maddox AS and Maddox PS (2018). LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching. Journal of Cell Biology 217:1869-1882.

Martinez P, Allsman LA, Brakke KA, Hoyt C, Hayes J, Liang H, Neher W, Rui Y, Roberts AM, Moradifam A, Goldstein B, Anderson CT, Rasmussen C (2018). Predicting division planes of three-dimensional cells by soap-film minimization. Plant Cell 30:2255-2266.

Dickinson DJ, Schwaeger F, Pintard L, Gotta M, and B Goldstein (2017). A Single-Cell Biochemistry Approach Reveals PAR Complex Dynamics during Cell Polarization. Developmental Cell 42:416-434.

hypsibius exemplaris Boothby water bear IDPs

Boothby TC, Tapia H, Brozena AH, Piszkiewicz S, Smith AE, Giovanninni I, Rebecchi L, Pielak GJ, Koshland D, and B Goldstein (2017). Tardigrades use intrinsically disordered proteins to survive desiccation. Molecular Cell 65:975-984.

hypsibius exemplaris tardigrade brain Frank Smith

Smith FW, Bartels PJ, and Goldstein B (2017). A hypothesis for the composition of the tardigrade brain and its implications for panarthropod brain evolution. Integrative and Comparative Biology 57:546-559.

Smith FW and B Goldstein (2017). Segmentation in Tardigrada and diversification of segmental patterns in Panarthropoda. Arthropod Structure & Development 46:328-340.

Zallen JA and Goldstein B (2017). Cellular mechanisms of morphogenesis. Semin Cell Dev Biol 67:101-102.

Russell JJ, Theriot JA, Sood P, Marshall WF, Landweber LF, Fritz-Laylin L, Polka JK, Oliferenko S, Gerbich T, Gladfelter A, Umen J, Bezanilla M, Lancaster MA, He S, Gibson MC, Goldstein B, Tanaka EM, Hu C-K, and Brunet A (2017). Non-model model organisms. BMC Biology 15:55.

Goldstein B and Zallen JA (2017). Cell Polarity and Morphogenesis: new technologies and new findings. Molecular Biology of the Cell, 28:699-700.

Naegeli KM, Hastie E, Garde A, Wang Z, Keeley DP, Gordon KL, Pani AM, Kelley LC, Morrissey MA, Chi Q, Goldstein B, Sherwood DR (2017). Cell Invasion In Vivo via Rapid Exocytosis of a Transient Lysosome-Derived Membrane Domain. Developmental Cell 43:403–417.

Linden LM, Gordon KL, Pani AM, Payne SG, Garde A, Burkholder D, Chi Q, Goldstein B and Sherwood DR (2017). Identification of regulators of germ stem cell enwrapment by its niche in C. elegans. Developmental Biology 429:271-284.

Ladouceur AM, Ranjan R, Smith L, Fadero T, Heppert J, Goldstein B, Maddox AS, Maddox PS (2017). CENP-A and topoisomerase-II antagonistically affect chromosome length. Journal of Cell Biology 216:2645-2655.

Heppert JK, Dickinson DJ, Pani AM, Higgins CD, Steward A, Ahringer J, Kuhn JR, and B Goldstein (2016). Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system. Molecular Biology of the Cell 27:3385-3394. (and a spectrum viewer tool)

Tintori SC, Osborne Nishimura E, Golden P, Lieb JD, and Goldstein B (2016). A transcriptional lineage of the early C. elegans embryo. Developmental Cell 38:430-444.
(and an interactive data visualization tool)

Smith FW, Boothby TC, Giovannini I, Rebecchi L, Jockusch EL, and B Goldstein (2016). The compact body plan of tardigrades evolved by the loss of a large body region. Current Biology 26:224-229.

Marston DJ*, Higgins CD*, Peters KA, Cupp TD, Dickinson DJ, Pani AM, Moore RP, Cox AH, Kiehart DP, and Goldstein B (2016). MRCK-1 drives apical constriction in C. elegans by linking developmental patterning to force generation. Current Biology 26:2079-2089.

Sullivan-Brown J, Tandon P, Bird KE, Dickinson DJ, Tintori SC, Heppert JK, Meserve JH, Trogden KP, Orlowski SK, Conlon FL, and Goldstein B (2016). Identifying regulators of morphogenesis common to vertebrate neural tube closure and C. elegans gastrulation. Genetics 202:123-139.

Heppert JK and Goldstein B (2016). Remodelling germ cells by intercellular cannibalism. Nature Cell Biology 18:1267-1268.

Goldstein B and King N (2016). The Future of Cell Biology: Emerging Model Organisms. Trends in Cell Biology 11:818-824.

Goldstein B (2016). Sydney Brenner on the Genetics of Caenorhabditis elegans. Genetics 204:1-2.

Dickinson DJ and Goldstein B (2016). CRISPR-based methods for Caenorhabditis elegans genome engineering. Genetics 202:885-901.

Dickinson, DJ, AM Pani, JK Heppert, CD Higgins, and B Goldstein (2015). Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035-1049.

Das A, Dickinson DJ, Wood CC, Goldstein B, and Slep KC (2015). Crescerin uses a TOG domain array to regulate microtubules in the primary cilium. Mol Biol Cell 26:4248-64.

Boothby TC, Tenlen JR, Smith FW, Wang JR, Patanella KA, Osborne Nishimura E, Tintori SC, Li Q, Jones CD, Yandell M, Messina DN, Glasscock J, and Goldstein B (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. PNAS 112:15976-15981 and follow-up letter.

Goldstein, B and DP Kiehart (2015). Moving Inward: Establishing the Mammalian Inner Cell Mass. Developmental Cell 34:385-386.

Yumerefendi H, Dickinson DJ, Wang H, Zimmerman SP, Bear JE, Goldstein B, Hahn K, and Kuhlman B (2015). Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS One 10(6):e0128443.

Osborne Nishimura E, Zhang JC, Werts AD, Goldstein B, Lieb JD (2015). Asymmetric Transcript Discovery by RNA-seq in C. elegans Blastomeres Identifies neg-1, a Gene Important for Anterior Morphogenesis. PLoS Genetics 11(4):e1005117.

Sarkies P, Selkirk ME, Jones JT, Blok V, Boothby T, Goldstein B, Hanelt B, Ardila-Garcia A, Fast NM, Schiffer PM, Kraus C, Taylor MJ, Koutsovoulos G, Blaxter ML, and Miska EA (2015). Ancient and Novel Small RNA Pathways Compensate for the Loss of piRNAs in Multiple Independent Nematode Lineages. PLoS Biology 13(2):e1002061.

Goldstein B (2015). The Thrill of Defeat: What Francis Crick and Sydney Brenner taught me about being scooped. Nautilus online, Feb 26 2015.

Martin, A.C. and B. Goldstein (2014). Apical constriction: themes and variations on a cellular mechanism driving morphogenesis. Development 141:1987-1998.

Dickinson, D.J., J.D. Ward, D.J. Reiner and B. Goldstein (2013). Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nature Methods 10:1028-1034.

Tenlen, J.R., S. McCaskill and B. Goldstein (2013). RNA interference can be used to disrupt gene function in tardigrades. Development Genes and Evolution 223:171-181.

Peters EC, Gossett AJ, Goldstein B, Der CJ, Reiner DJ (2013). Redundant Canonical and Noncanonical Caenorhabditis elegans p21-Activated Kinase Signaling Governs Distal Tip Cell Migrations. G3 3:181-195.

Roh-Johnson, M.*, G. Shemer.*, C.D. Higgins, J.H. McClellan, A.D. Werts, U.S. Tulu, L. Gao, E. Betzig, D.P. Kiehart and B. Goldstein (2012). Triggering a Cell Shape Change by Exploiting Pre-Existing Actomyosin Contractions. Science 335:1232-1235.

Gao, L., L. Shao, C.D. Higgins, J.S. Poulton, M. Peifer, M.W. Davidson, X. Wu, B. Goldstein and E. Betzig (2012). Noninvasive imaging of 3D dynamics in thickly fluorescent specimens beyond the diffraction limit. Cell 151:1370-1385.

Sullivan-Brown, J. and B. Goldstein (2012) Neural tube closure: The curious case of shrinking junctions. Current Biology 22:R574-R576.

Edgar, L.G. and B. Goldstein (2012). Culture and Manipulation of Embryonic Cells, in Caenorhabditis elegans: Cell Biology and Physiology, eds. Joel H. Rothman and Andrew Singson. Methods in Cell Biology 107:151-175.

Tse, Y.C., M. Werner, K.M. Longhini, J.-C. Labbé, B. Goldstein and M. Glotzer (2012) RhoA activation during polarization and cytokinesis of the early C. elegans embryo are differentially dependent on NOP-1 and CYK-4. Molecular Biology of the Cell 23:4020-4031.

Werts, A.D., M. Roh-Johnson and B. Goldstein (2011) Dynamic localization of C. elegans TPR-GoLoco proteins mediates mitotic spindle orientation by extrinsic signaling. Development 138:4411-4422.

Sawyer, J.M., S. Glass, T. Li, G. Shemer, N.D. White, N.G. Starostina, E.T. Kipreos, C.D. Jones, and B. Goldstein (2011) Overcoming Redundancy: an RNAi Enhancer Screen for Morphogenesis Genes in Caenorhabditis elegans. Genetics 188:549-564.

Goldstein, B. (2011) Primer: Visualizing with ImageJ. Make 27:116-121. This is a magazine article that introduces non-scientists to ImageJ and encourages hobbyists to write new ImageJ plugins that might benefit science. Here's a more recent verison – instructions need updating periodically for this primer to keep working on newer operating systems.